BREAST CENTER REAL-TIME PCR CORE LABORATORY
The Breast Center Real-Time PCR Core Facility has been in operation since 1999, and the Core Facility updated the services to support the molecular biological applications in research and clinical activities of the Breast Center at Baylor College of Medicine.
The Core Facility, located on the 11th floor of the Alkek building, houses an ABI Prism 7700 Sequence Detection System. This instrument combines thermal cycling, fluorescence detection, and application-specific software to detect the accumulation of target product cycle-by-cycle during PCR. Using fluorescent probes (TaqMan) or SYBR Green, the 7700 system can provide real-time quantitation of DNA or RNA by monitoring the exponential phase of the PCR reaction. Applications for real-time PCR technology include quantitative gene expression analysis, pathogen detection/quantitation, allelic discrimination (SNP detection), and mutation screening.
Breast Center Real-time PCR Core Facility is overseen by Dr. Powel Brown and is managed by Heetae Kim. Various services offered by the Core are available to users on a fee for service basis, and can be requested via e-mail (heetaek@breastcenter.tmc.edu) or the forms available at this web site. Each user must sign up in advance and supply a valid account number before being allowed access to the instrument. Non-University users may also take advantage of the Breast Center Core Facility on a fee for service basis.
First time user must undergo basic training session offered by the Core Manager before being allowed access to the instrument; training sessions will be arranged on a per need basis, free of charge.
APPLICATIONS
Quantitative Gene
Expression
Allelic Discrimination
Mutation Screening
SERVICES & FEES
New Assay
Existing Assay
Existing Assay List
Do-It-Yourself Assay
Order Form
First-time User
