Breast Center Real-Time PCR Core Facility

Existing PCR Assay List

Total existing assays: 91

Human assays: 82
Mouse assays: 9

Transcript	Species	Synonym	Description	Accession
ABC-1	human	ABCA-1	ATP-binding cassette sub-family A member 1	NM_005502
ACS1	human		acyl-CoA synthetase long-chain family member 1	NM_001995
Adipsin	human		complement factor D preproprotein	NM_001928
AIB1	human	BCAS1	breast carcinoma amplified sequence 1	NM_003657
AQP3	human		aquaporin 3	NM_004925
BCL2	human		B-cell lymphoma protein 2 alpha isoform	NM_000633
B-myb	human		B-myb gene	X13293
Brd2	human		bromodomain containing protein 2	NM_005104
Brd3	human		bromodomain containing protein 3	NM_007371
BTG2	human		B-cell translocation gene 2	NM_006763
CA12	human		carbonic anhydrase XII isoform 2 precursor	AF037335
Cathepsin D	human		cathepsin D preproprotein	NM_001909
cFos	human		cellular oncogene c-fos	V01512
cJun	human		c-jun proto oncogene	J04111
cJun+Tam67	human
cMyc	human		v-myc myelocytomatosis viral oncogene homolog	NM_002467
COX-2	human	PTGS2	prostaglandin-endoperoxide synthase 2 precursor	NM_000963
COX-2	mouse		prostaglandin-endoperoxide synthase 2 precursor	NM_011198
CXCL12	human		chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)	NM_000609
Cyclin D1	human		cyclin D1	cyclin D1
Cyclin E*	human
EGR3	human		early growth response 3	NM_004430
EGFR	human		epidermal growth factor receptor	NM_005228
EMS1	human		amplaxin (EMS1) mRNA	M98343
ErbB2	human		v-erb-b2 erythroblastic leukemia viral oncogene	X03363
ErbB3	human		epidermal growth factor receptor (ERBB3)	M29366
ErbB4	human		v-erb-a erythroblastic leukemia viral oncogene	L07868
ERa	human		estrogen receptor 1	NM_000125
ERb	human		estrogen receptor 2 (ER beta)	AB006590
E2IG1	human	HSPB8	heat shock 22kDa protein 8	NM_014365
Fibronectin	human		Fibronectin (migration-stimulating factor)	AL832202
FosB	human		FBJ murine osteosarcoma viral oncogene homolog	NM_006732
Fra1	human		FOS-like antigen 1	NM_005438
Fra2	human		FOS-like antigen 2	NM_005253
GST Pi	human		anionic glutathione-S-transferase	X15480
24 Hydroxylase	human		cytochrome P450, family 24, subfamily A, polypeptide 1	NM_000782
ID-1	human		inhibitor of DNA binding 1	XM_046179
IGFBP1	human		insulin-like growth factor binding protein 1	NM_000596
IGFBP2	human		insulin-like growth factor binding protein 2	NM_000597
IGFBP3	human		insulin-like growth factor binding protein 3	NM_000598
IGFBP4	human		insulin-like growth factor binding protein 4	NM_001552
IGFBP5	human, mouse		insulin-like growth factor binding protein 5	M62782
IGFBP6	human		insulin-like growth factor binding protein 6	NM_002178
JunB	human		jun B proto-oncogene	NM_002229
JunD	human		jun D proto-oncogene	NM_005354
5 LOX	human		arachidonate 5-lipoxygenase	NM_000698
15 LOX	human		arachidonate 15-lipoxygenase	NM_001140
LXRa	human		liver X receptor alpha	NM_005693
LXRb	human		liver X receptor beta	NM_007121
LXRb	mouse		liver X receptor beta
Maspin	human		protease inhibitor 5 (maspin)	NM_002639
MMP1	human		matrix metalloproteinase 1 preproprotein	NM_002421
MMP9*	human		matrix metalloproteinase 9 preproprotein	NM_004994
Mucin	human		polymorphic epithelial mucin (PEM)	J05581
Nur77	human		nuclear receptor subfamily 4 group A member 1	L13740
ODC	mouse		ornithine decarboxylase, structural (Odc)	NM_013614
Osteopontin	human	SPP1	secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)	NM_000582
PCNA	human		proliferating cell nuclear antigen	XM_045513
PPARg	human		peroxisome proliferactor activated receptor gamma	X90563
PR	human		progesterone receptor (PR)	AF016381
PSA	human		prostate specific antigen(KLK3)	XM_031767
pS2	human		presenilin 2 isoform	NM_000447
pS2 5'flank*	human
p21	human		cyclin-dependent kinase inhibitor 1A	NM_078467
p27	human, mouse		cyclin-dependent kinase inhibitor 1B	NM_004064
RA-OH	human		retinoic acid hydroxylase	AF005418
RARa	human		retinoic acid receptor, alpha	XM_049946
RARb	human		retinoic acid receptor beta 4	AF157483
RARg	human		retinoic acid receptor gamma-2	X57280
RXRa	human		retinoid X receptor, alpha	NM_002957
RXRb	human		retinoid X receptor, beta	XM_042578
RXRg	human		retinoid X receptor-gamma	U38480
SCD-1	human		stearoyl-CoA desaturase (SCD)	AF097514
SOD-Mn	human		superoxide dismutase 2, mitochondrial (SOD2	NM_000636
SREBP-1	human		sterol regulatory element binding transcription	NM_004176
Survivin	human		baculoviral IAP repeat-containing 5 (survivin)	NM_001168
TGFbRIII*	human
TIMP1	human		tissue inhibitor of metalloproteinase 1	NM_003254
TTgase	human		transglutaminase 2 isoform a	NM_004613
TOB	human		transducer of ERBB2	NM_005749
VDR	human		vitamin D (1,25- dihydroxyvitamin D3) receptor	NM_000376

(Internal Control)
b-Actin	human, mouse			BE547581
Cyclophilin	human, mouse			AA384590
36B4	human			BF103761
18S	human, mouse, rat			AF397158